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TSAP activity was assayed by counting the number of colony forming units (cfu) obtained from plasmid that had been digested, dephosphorylated and religated.
Figure 1, Panel B, demonstrates there is no difference in banding pattern between the 15-minute incubation and the extended 2-hour incubation. Gel analysis of the Streamlined Restriction Digestion, Dephosphorylation and Ligation reaction using Promega restriction enzymes and digestion buffers.
Sequence Manipulation Suite A collection of web based programs for analyzing and formatting DNA and protein sequences.
Expasy A web based collection of tools for protein sequence analysis and structure prediction.
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TSAP Thermosensitive Alkaline Phosphatase is active in all Promega restriction enzyme buffers, making it an ideal choice for convenient, rapid dephosphorylation of DNA.